- Antigen-presenting cells and inflammatory response
- Pharmacogenomics
- Physiology of Inflammatory processes
- Experimental Oncology
- Genetics of Lymphoid Malignancies
- Hematological genetics
- Molecular Genetics of Hemophilia
- Haemostasis and thrombosis
- Immunology of Respiratory Diseases
- Experimental immunology
- Innate immunity
- Onco-immunology
- Mutagenesis
- Pathogenesis and inmunology of infectious processes
- Pathogenesis of viral infections
- Trombosis Experimental e Inmunobiología de la Inflamación
The objective of the laboratory is to study genetic and epigenetic alterations in hematological disorders, mainly of myeloid strain, tending to a better understanding of their biological behavior and the development of techniques that contribute to their diagnosis, prognosis, and/or therapeutic selection.
Chair: Irene Larripa, PhD
Supirior investigator – CONICET
E-mail: ibl@hematologia.anm.edu.ar
Researchers
- Carolina Belli, PhD. Associated investigator – CONICET
Fellows
- María Fernanda Camacho Rodriguez, MSc. PhD student – ANPCyT
- Carla Orellana, MSc. PhD student, ANM scholarship
- María Jazmin Toloza, MSc. PhD student – CONICET
- Marco Lincango Yupanki, Ing. PhD student – ANPCYT
Research lines
Philadelphia (Ph) positive leukemias, Ph negative myeloproliferative neoplasms, myelodysplastic syndromes, acute myeloid leukemia, and spinal cord failure
- To study the response to treatment with tyrosine kinase inhibitors evaluating the dynamics of BCR-ABL1 transcripts.
- To determine resistance mechanisms dependent and independent of BCR-ABL1.
- To establish genotype-phenotype correlations and treatment impact concerning the allelic load of JAK2V617F.
- To determine susceptibility markers, including polymorphisms in cytokine genes, in the PD-L1 programmed death-ligand or repair machinery.
- To analyze the immune component involved, evaluating polymorphisms, cytokine expression levels, and lineage-specific transcription factors.
- To evaluate genetic alterations (cytogenetic alterations and mutations in epigenetic regulators, splicing machinery, transcription factors, among others) and epigenetic (aberrant methylation) as etiopathogenic mechanisms and clonal progression.
- To determine the clinical implication of the analyzed variants and corroborate whether they influence the response to treatment, survival, or cellular metabolism to establish algorithms that collaborate in clinical and therapeutic decisions.
- To identify helpful prognostic factors in risk stratification and response to treatment thanks to integration in multicenter studies.
Publications
- Myelodysplastic syndromes in South America: a multinational study of 1080 patients. Belli CB, Pinheiro RF, Bestach Y, Larripa IB, da Silva Tanizawa RS, Alfonso G, Gonzalez J, Rosenhain M, Watman N, Cavalcante de Andrade Silva M, Negri Aranguren P, García Rivello H, Magalhaes SM, Valladares X, Undurraga MS, Velloso ER. Am J Hematol. 2015 Oct;90(10):851-8. doi: 10.1002/ajh.24097. Epub 2015 Sep 10.
- BCR-ABL mutations in chronic myeloid leukemia treated with tyrosine kinase inhibitors and impact on survival. Pagnano KB, Bendit I, Boquimpani C, De Souza CA, Miranda EC, Zalcberg I, LarripaI, Nardinelli L, Silveira RA, Fogliatto L, Spector N, Funke V, Pasquini R, Hungria V, Chiattone CS, Clementino N, Conchon M, Moiraghi EB, Lopez JL, Pavlovsky C, Pavlovsky MA, Cervera EE, Meillon LA, Simões B, Hamerschlak N, Bozzano AH, Mayta E, Cortes J, Bengió RM; Latin American Leukemia Net (LALNET). Cancer Invest. 2015;33(9):451-8. doi: 10.3109/07357907.2015.1065499. Epub 2015 Aug 17.
- Polymorphic variants of GSTM1, GSTT1, and GSTP1 genes in childhood acute leukemias: A preliminary study in Argentina. Weich N, Nuñez MC, Galimberti G, Elena G, Acevedo S, Larripa I, Fundia AF. Hematology. 2015 Oct;20(9):511-6. doi: 10.1179/1607845415Y.0000000007. Epub 2015 Mar 23.
- Polymorphisms in TNF and IFNG are associated with clinical characteristics of aplastic anemia in Argentinean population. Bestach Y, Sieza Y, Attie M, Riccheri C, Verri V, Bolesina M, Bengió R, Larripa I, Belli C. Leuk Lymphoma. 2015 Jun;56(6):1793-8. doi: 10.3109/10428194.2014.966707. Epub 2015 Jan 21.
- Clinical activity of ponatinib in one patient with chronic myeloid leukemia in chronic phase with e19a2 transcript and T315I mutation. Ferri CA, Bianchini M, Bengió RM, Moiraghi EB, Gonzalez MS, Noriega MF, LarripaIB. Eur J Haematol. 2015 Mar;94(3):270-2. doi: 10.1111/ejh.12358. Epub 2014 May 13.
- CAMKIIγ, HSP70 and HSP90 transcripts are differentially expressed in chronic myeloid leukemia cells from patients with resistant mutated disease. Gonzalez M, De Brasi C, Ferri C, Bengió R, Bianchini M, Larripa I. Leuk Lymphoma. 2014 Sep;55(9):2101-8. doi: 10.3109/10428194.2013.861070. Epub 2014 Mar 7
- New mutation L324M in the ABL1 kinase domain: does it confer high resistance to second-generation inhibitors? Noriega MF, Ferri CA, Icardi G, Bullorsky E, Korin J, Larripa I. Leuk Lymphoma. 2014 Mar;55(3):698-701. doi: 10.3109/10428194.2013.810735. Epub 2013 Jul 22. No abstract available.
- Glutathione S-transferase gene polymorphisms in celiac disease and their correlation with genomic instability phenotype. Fundia AF, Weich N, Crivelli A, La Motta G, Larripa IB, Slavutsky I. Clin Res Hepatol Gastroenterol. 2014 Jun;38(3):379-84. doi: 10.1016/j.clinre.2014.01.007. Epub 2014 Feb 22.
- New mutation L324M in the ABL1 kinase domain: does it confer high resistance to second-generation inhibitors? Noriega MF, Ferri CA, Icardi G, Bullorsky E, Korin J, Larripa I. Leuk Lymphoma. 2014 Mar;55(3):698-701. doi: 10.3109/10428194.2013.810735. Epub 2013 Jul 22. No abstract available.
- Application of the revised International Prognostic Scoring System for myelodysplastic syndromes in Argentinean patients. Belli CB, Bestach Y, Giunta M, Iastrebner M, Santos I, Pintos N, Arbelbide J, Basquiera AL, Bengió R, Larripa I. Ann Hematol. 2014 Apr;93(4):705-7. doi: 10.1007/s00277-013-1848-4. Epub 2013 Jul 28. No abstract available.
- Improved diagnosis of the transition to JAK2 (V⁶¹⁷F) homozygosity: the key feature for predicting the evolution of myeloproliferative neoplasms. Gonzalez MS, De Brasi CD, Bianchini M, Gargallo P, Stanganelli C, Zalcberg I,Larripa IB. PLoS One. 2014 Jan 27;9(1):e86401. doi: 10.1371/journal.pone.0086401. eCollection 2014.
- Expression of LYN and PTEN genes in chronic myeloid leukemia and their importance in therapeutic strategy. Ferri C, Bianchini M, Bengió R, Larripa I. Blood Cells Mol Dis. 2014 Feb-Mar;52(2-3):121-5. doi: 10.1016/j.bcmd.2013.09.002. Epub 2013 Oct.
- Early detection and quantification of mutations in the tyrosine kinase domain of chimerical BCR-ABL1 gene combining high-resolution melting analysis and mutant-allele specific quantitative polymerase chain reaction. Ferri C, Bianchini M, Icardi G, Belli C, Bengió R, Larripa I. Leuk Lymphoma. 2013 Mar;54(3):598-606. doi: 10.3109/10428194.2012.718767. Epub 2012 Aug 31.